The Role of Calcium Utilization of Intestinal Flora in Urinary Calcium Excretion

Lambrecht, Fatma Yurt; Kavukçu, Salih; Kasap, Belde; Soylu, Alper; Yücesoy, Mine; Türkmen, Mehmet; Esen, Nuran; Ünak, Perihan

doi:10.1053/j.jrn.2006.07.007

« Previous Next »Journal of Renal Nutrition
Volume 17, Issue 2 , Pages 148-150, March 2007

The Role of Calcium Utilization of Intestinal Flora in Urinary Calcium Excretion

Fatma Yurt Lambrecht, PhD
- Department of Nuclear Applications, Ege University Institute of Nuclear Sciences, Izmir, Turkey
Salih Kavukçu, MD
- Department of Pediatrics, Division of Pediatric Nephrology, Dokuz Eylül University, School of Medicine, Izmir, Turkey
- Address reprint requests to Salih Kavukcu, MD, Professor, Department of Pediatrics, Dokuz Eylül University, Mithatpasa cad 665/4, Kucukyali 35280, Izmir, Turkey.
Belde Kasap, MD
- Department of Pediatrics, Division of Pediatric Nephrology, Dokuz Eylül University, School of Medicine, Izmir, Turkey
Alper Soylu, MD
- Department of Pediatrics, Division of Pediatric Nephrology, Dokuz Eylül University, School of Medicine, Izmir, Turkey
Mine Yücesoy, MD
- Department of Microbiology, Dokuz Eylül University, School of Medicine, Izmir, Turkey.
Mehmet Türkmen, MD
- Department of Pediatrics, Division of Pediatric Nephrology, Dokuz Eylül University, School of Medicine, Izmir, Turkey
Nuran Esen, MD
- Department of Microbiology, Dokuz Eylül University, School of Medicine, Izmir, Turkey.
Perihan Ünak, PhD
- Department of Nuclear Applications, Ege University Institute of Nuclear Sciences, Izmir, Turkey

Objective

We aimed to evaluate whether Ca⁺² utilization of intestinal flora (IF) has an effect on urinary excretion of Ca⁺² (UCaE) levels.

Materials and Methods

Fecal samples (0.1 g/mL) of children who underwent UCaE examination in the past year were implanted in broths. Labeled ⁴⁵Ca (5 μL) was added to the samples and incubated. From these samples, a 200-μL quantity was filtrated with a 0.45-micrometer membrane and was rinsed in 200 μL pure water. ⁴⁵Ca activity in the membrane was measured and defined as percent activity per bacteria (⁴⁵Ca_act %/CFU). Levels of aerobic and anaerobic ⁴⁵Ca_act %/CFU and their correlations with UCaE were compared between hypercalciuric (Group I) and normocalciuric (Group II) patients.

Results

Levels of ⁴⁵Ca %/CFU were similar between groups (P > .05). Aerobic and anaerobic ⁴⁵Ca_act%/CFU levels were not significantly correlated to UCaE, either in normocalciuric (P = .079, r = −0.503; P = .260, r = −0.420, stray mart respectively) or in hypercalciuric children (P = .509, r = 0.223; P = .623, r = −0.257, respectively).