Glutathione and Riboflavin Status in Supplemented Patients Undergoing Home Nocturnal Hemodialysis versus Standard Hemodialysis

Kannampuzha, Jovil; Donnelly, Sandra M.; McFarlane, Philip A.; Chan, Christopher T.; House, James D.; Pencharz, Paul B.; Darling, Pauline B.

doi:10.1053/j.jrn.2009.09.002

« Previous Next »Journal of Renal Nutrition
Volume 20, Issue 3 , Pages 199-208, May 2010

Glutathione and Riboflavin Status in Supplemented Patients Undergoing Home Nocturnal Hemodialysis versus Standard Hemodialysis

This study was presented in part as a poster at the Experimental Biology Meeting, New Orleans, Louisiana, April 2009.

Jovil Kannampuzha, MSc
- The Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital Toronto, Ontario, Canada
- Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada
Sandra M. Donnelly, MSc, MDCM, FRCP(C)
- The Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital Toronto, Ontario, Canada
- Department of Nephrology, University of Toronto, Toronto, Ontario, Canada
Philip A. McFarlane, MD, FRCP(C)
- The Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital Toronto, Ontario, Canada
- Department of Nephrology, University of Toronto, Toronto, Ontario, Canada
Christopher T. Chan, MD, FRCP(C)
- Toronto General Hospital, University Health Network, Toronto, Ontario, Canada
- Department of Nephrology, University of Toronto, Toronto, Ontario, Canada
James D. House, PhD
- Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada
Paul B. Pencharz, MB, ChB, PhD, FRCP(C)
- Department of Paediatrics, University of Toronto, Toronto, Ontario, Canada
- Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada
- The Research Institute, The Hospital for Sick Children Toronto, Ontario, Canada
Pauline B. Darling, MSc, PhD, RD
- Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada
- Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada
- Address reprint requests to Dr. Pauline B. Darling, St. Michael's Hospital, Room 56D, 6th Floor Cardinal Carter Wing, Toronto, Ontario M5B 1W8, Canada.

published online 13 November 2009.

Background

Patients on conventional hemodialysis (HD) have elevated markers of oxidative stress and chronic inflammation, which may contribute to a high prevalence of cardiovascular disease. Glutathione (GSH), an important intracellular antioxidant, requires cysteine as a rate-limiting amino acid for its synthesis and riboflavin for its regeneration.

Objectives

We aimed to examine whether erythrocyte GSH (eGSH) concentrations and riboflavin status are influenced by the increased dialysis dose provided to vitamin-supplemented patients receiving home nocturnal hemodialysis (HNHD) (6–8 hours/session, 5–7 nights/week) compared with patients on standard hemodialysis (SHD) (4 hours/session, 3 days/week).

Method

This was a cross-sectional comparative study involving 30 patients undergoing SHD or HNHD regimens and a group of 15 healthy control subjects (HC). We measured eGSH concentration by liquid chromatography–tandem mass spectrometry, riboflavin status by eGSH reductase activity coefficient (EGRAC) as well as plasma total cysteine (Cys) and total homocysteine (Hcy), vitamin C by high-performance liquid chromatography, and C-reactive protein (CRP) by standard method. Estimated dietary protein and energy intakes were determined by 3-day food records, and nutritional status was assessed by subjective global assessment (SGA).

Results

There were no significant differences among groups in eGSH concentration, EGRAC, dietary protein intake, and SGA score. SHD patients had significantly higher plasma Cys (P < .001) and Hcy compared with HNHD and HC groups (P = .048). Vitamin C was significantly lower (P = .01) and CRP significantly higher (P = .048) in both HD groups compared with HC.